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- Serum IL6 and sIL6R were measured by Elisa assay inall subjects. 采用Elisa法测定3组的血清IL-6、sIL-6R浓度。
- The pepsin contents of gastric juice were determined by JacalinHRP ELISA assay. 方法:采用HRP标记jacalin,通过elisa法检测各种胃病患者胃液中的胃蛋白酶。
- Methods we detected the changes of antigen specific AFC and sIgA in in- testinal mucosa by ELISPOT and ELISA assay. 方法采用 ELISPOT 和 ELISA 法检测了胃粘膜、PP 结抗原特异性形成细胞(AFC)和小肠粘液 sIgA。
- The result showed that heterologous assay, especially heterology in structure of hapten would largely improve the sensitivity of ELISA assay. 结果表明:异源分析,特别是半抗原结构异源可大幅度提高检测灵敏度;
- Western blot analysis was used to detect pl85 HER-2, HER-2 ECD and phospho-HER-2. Two-site ELISA assay was used for the detection of HER-2 ECD. 双抗夹心ELISA法检测细胞培养上清中HER-2 ECD水平,Western blot检测细胞HER-2蛋白水平。
- Autoantibodies against mTie-2 were found in sera of mice immunized with chTie-2 in Western blotting analysis and ELISA assay. 同时我们用小鼠自身Tie-2受体相应片段(mTie-2)的重组蛋白作为同种对照。
- The result showed that heterologous assay,especially heterology in structure of hapten would largely(improve) the sensitivity of ELISA assay. 结果表明:异源分析,特别是半抗原结构异源可大幅度提高检测灵敏度;
- Method: Serum sIL?2R level was measured with ELISA assay in 70 patients with different liver diseases and 22 healthy persons. 方法:采用双抗体夹心ELISA法检测慢性乙型肝炎、肝硬化和原发性肝癌患者(共70例)与健康人(22例)的外周血sIL?2R水平,并比较原发性肝癌(15例)手术前后sIL?2R水平的变化。
- Methods:Serum sIL 2R level was detected in 38 patients(12 detected after treatment) and 35 healthy individuals by using sandwish ELISA assay. 方法:采用ELISA法测定38例肝硬变患者(12例经治疗后)和35名正常人血清中sIL?2R含量。
- Method Antigangliosides GM1 antibody and cephalin antibody in serum (n=28) and cerebrospinal fluid (n=18) were examind in MS patients with ELISA assay. 方法采用ELISA方法测定多发性硬化患者活动期血清(28例)和脑脊液(18例)的GM1抗体、脑磷脂抗体和髓鞘碱性蛋白。
- The in vitro and in vivo levels of mIL-12 and the in vivo activity of cytotoxic T lymphocyte(CTL)were examined by ELISA assay and modified LDH release assay,respectively. ELISA法检测细胞上清及小鼠血液中mIL-12水平,LDH释放法检测小鼠体内细胞毒性T淋巴细胞(CTL)杀伤活性。
- The inhibition of HIV-1 Tat/PCAF BRD interaction in vitro was determined by ELISA assay,and some influential factors of biological activity were discussed. ELISA检测法测定了它们体外抑制HIV-1Tat/PCAFBRD的活性;并对影响活性的因素进行了讨论.
- Methods Urinary pyridinoline and deoxypyridinoline were measured by ELISA assay in 26 patients with local cancer and 69 advanced cancer patients with or without bone metastases. 方法采用ELISA方法检测了26例局限性恶性肿瘤、35例进展期肿瘤骨转移阴性和34例骨转移阳性的尿吡啶酚和脱氧吡啶酚水平。
- Medthod:Urine pyridinoline and deoxypyridinline were measured by ELISA assay in 26 patients with local cancer and 69 advanced cancer patients with or without bone metastases. 方法:采用ELISA方法检测了26例限局性恶性肿瘤、35例进展期肿瘤骨转移阴性和34例骨转移阳性的尿吡啶酚和脱氧吡啶酚水平。
- Objective To appraise the two methods[polyethylene glycol(PEG) precipitation assay and CIC-C1q ELISA assay] which were used to detect the circulating immune complexes(CIC) and the clinical significance of CIC. 目的 对测定血清循环免疫复合物 ( circulating immune complexes,CIC)的两种方法 ( PEG沉淀法及 CIC-C1 q ELISA法 )进行评价 ,进一步探讨 CIC测定的临床意义。
- Objective: To investigate the regulation of MMP-9/TIMP-1 expressions in glyceryl trinitrate (GTN), that acts as the donor of nitric oxide, treated Hela cells with microarray technique, RT-PCR and ELISA assay. 目的利用基因芯片、RT-PCR技术和ELISA方法观察硝酸甘油(GTN)作为一氧化氮供体对人宫颈癌细胞(Hela)MMP-9/TIUP-1基因及蛋白表达的调控。
- Immunocytochemical staining showed significant staining of the esophageal cancer line Eca109. The result of cell ELISA assay revealed that soluble scFv had highly specific and could combined with Eca109 cells, but not with BGC-823 and NHEEC. 细胞ELISA测定结果显示可溶性抗体具有较高的特异性,能与Eca109细胞结合,而不与胃癌BGC-823和NHEEC结合。
- In order to prevent cross linking reaction happening between BSA moleculars, the modified bovine serum albumen (M-BSA) was used in the EDC catalytic coupled reaction between AFB_1 and BSA, the coupled reaction was monitored by ELISA assay. 在第二步EDC法制备AFB_1肟化物与载体蛋白的偶联物时,采用修饰的牛血清白蛋白(M-BSA),避免了EDC引起的蛋白质自身交联问题。
- After transfection of pPTA1/Ig into COS 7 cells by DEAE dextran, expression of a secreting fusion protein was identified by affinity chromatography and sandwich ELISA assay with anti PTA1 mAb and HRP anti hIgFc mAb. 重组载体通过DEAE?dextran法转染COS?7细胞,经夹心ELISA及亲和层析、SDS?PAGE鉴定融合蛋白的表达及免疫学活性。
- Methods: DGR with 99MTC-EHIDA radionuclide imaging,serum NO concentration with colorimetric assay, H.pylori infection with histology (Giemsa stain) and H.pylori-IgG level with ELISA assay were detected in 65 patients with chronic gastritis. 方法 :对 6 5例慢性胃炎患者 ;用同位素99MTC -EHIDA测定十二指肠胃胆汁反流 ;硝酸还原酶法测定血清NO含量 ;采用胃黏膜经Giemsa染色后检测H .;pylori;用ELISA法测定血清H
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