Immunocytochemical staining showed significant staining of the esophageal cancer line Eca109. The result of cell ELISA assay revealed that soluble scFv had highly specific and could combined with Eca109 cells, but not with BGC-823 and NHEEC.

• 细胞ELISA测定结果显示可溶性抗体具有较高的特异性,能与Eca109细胞结合,而不与胃癌BGC-823和NHEEC结合。