We chose 3 up-regulated proteins: vimentin, carbonic anhydrase (CA) and 14-3-3 protein for immunohistochemistry and RT-PCR, the results could confirm the reliability of our proteomic research.

• 在9个表达上调的蛋白质中，选取vimentin（波形蛋白）、碳酸酐酶（Carbonic anhydrase，CA）和14-3-3蛋白进行免疫组化和RT-PCR验证，结果显示这三个蛋白质在细胞和mRNA水平上呈阳性表达，与蛋白质组学实验结果相符。