Total protein were de Lermined by absorbance from hydrolysis with ninhydrin and pure protein were analyzed by differance of absorbance from hydrolysis and unhydrolysis all samples methanol extracts with ninhydrin.

• 与未水解的标样及饲料样品的甲醇提取液的茚三酮反应吸收值之差计算去游离氨基酸的纯蛋白质量。