Though endosperm from 6-16DPA(day post anthesis) grain could all be used for the assay of specificgene expression, 8-10 DPA endosperm were found to be most suitable.

• 剥离当天的小麦胚乳6-16DPA(day post anthesis)可以用于基因表达活性检测，但以 8-10DPA 为最好，过嫩或过老的胚乳细胞活性明显降低，影响 GUS 表达；