The virus fluid of high content had been inactivated by 0.3% formaldehyde solution and emulsified by oil emulsion adjuvants, whose TCID50 was for 10~(-7.1934)/0.1ml, then the inactivated oil emulsion vaccine against DRV came into being.

• 用番鸭胚成纤维细胞传代培养，获得病变集中、一致、病毒含量较高(TCID_(50)为10~(-7.；1934)/0