The two groups continued to be cultured for 24 and 36 hours with ACTH stimulation. The concentrations of aldosterone and cortisol at 24 and 36 hours were detected respectively.

• 加入促肾上腺皮质激素 (ACTH)继续培养 2 4、36 h,用放免法分别测 ACTH刺激前后的醛固酮、皮质醇浓度。