The results showed that the cotyledon cultured for 3 days has the highest regeneration frequency of 95.5%,when it was placed in the MS basic medium supplemented with BA0.4mg/L. Furthermore,adventitious bud was appeared after 12 days culture.

• 结果表明:以MS为基本培养基；附加BA0.；4mg/L处理时；3d苗龄的子叶的分化频率最高；可达95