The result of ANS binding fluorescence indicated that the hydrophobic surface of the mutant R283G and H287A were all decreased compared to the wild-type AK, while hydrophobic surface of the mutant C274A has almost no change.

• ANS荧光光谱显示突变体R283G和H287A的疏水表面相对于野生型大大降低,而突变体C274A则和野生型基本没有差别。