The recombinant plasmid containing ATF-PAI2CD fused gene, pZWY-ATF-PAI2CD, was transferred to the competent cells of yeast strain GS115. After the phenotype selection, the positive clones were induced with methanol to express ATF-PAI2CD.

• 该质粒转化毕赤酵母菌GS115 ；用G4 18 YPD平板筛选高拷贝转化子 ；然后用甲醇诱导表达 .；工程菌用摇瓶发酵；表达产物ATF PAI2CD占培养液中总蛋白 5 0%25以上