The decalcified tissue blocks were sectioned in a mesial-distal plane, then stained with hematoxylin and eosin(HE).

• 10%25甲醛心脏灌注固定，待组织固定后，取大鼠上颌骨组织，包括牙及牙槽骨，制备近远中组织切片，HE染色，应用计算机图像分析软件量化根分叉区形态学改变情况，将所有结果进行统计学分析。