The coupling ratio between monoclonal antibodies and Sepharose 4B is 85.21%. The active groups on the Sepharose 4B base materials are blocked and washed,stored.

• 取经溴化氰活化的Sepharose 4B与单克隆抗体抗体偶联；偶联比为85.；21%25；封闭活化位点；洗涤；制备IAC柱。