The VEGF mRNA and protein expression in rAAV2/hVEGF165-tranduced cardiomyocytes were examined by RT-PCR and ELISA at different time (0, 24, 48 and 72 hours) under hypoxic condition, and the normoxic culture group was used as control.

• 对心肌细胞进行低氧培养,并于培养的0、24、48、72h,分别应用RT-PCR技术检测VEGF165 mRNA表达、ELISA法检测hVEGF165蛋白的水平,并与对照组(予以常氧培养)进行比较。