The ScFvA1-Etag and the glucose-linker-ScFvA1 (GLS) fusion gene were cloned into the vector pPIC9K and expressed in methylotrophic yeast Pichia pastoris GS115, respectively, under the control of the AOX1 promoter.

• 将ScFvA1-Etag基因，GLS融合基因重组进大肠杆菌-酵母穿梭质粒pPIC9K, 分别构建含有ScFvA1-Etag基因，GLS融合基因的表达载体pPIC9K-ScFvA1-Etag，pPIC9K-GLS。