Pull-down and coimmunoprecipitation were used to confirm this interaction.We also mapped the interaction regions and discovered that CtBP2 and CCNH colocalized in nucleus.

• 我们利用体外及体内结合实验，验证了二者之间的相互作用，确定了结合的区域，并且首次发现CtBP2与CCNH共定位于细胞核。