Optimum pH of assay for serum AAP was 8.0,concentration of Tris HCl buffer was 150 mmol/L,the final concentration of Ala pNA was 3 mmol/L. Michel Meten constant(Km)was 0.74 mmol/L. Absorbency increased along with time in 8 minutes at least.

• 酶反应最适pH8 0 ,Tris HCl缓冲液的最佳浓度 15 0mmol/L ,Ala pNa浓度以 3mmol/L为宜 ,米氏常数 (Km) 0 74mmol/L ,在 8min观测时间内吸光度变化与时间成比例 ,线性范围可达 180 0U/L ,平均批内、批间CV分别为 1 0 4%25和 2 0 2%25。