N. coenophialum and N. lolii could be distinguished effectively via PCR amplification by using two pairs of primers FM1/R1 and F3/R3 designed on the basis of AY191830 in GenBank.

• 根据GenBank上AY191830的序列设计引物FM1/R1、F3/R3，两对引物分别能有效地区分N.；coenophialum和N