More hydrophilic peptide could be eluted by polarer solvents efficiently.Its recovery rate could be improved by increasing trifluoroacetic acid wash times.

• 结果表明，洗脱溶剂对多肽的洗脱效果与多肽及溶剂的极性有关：亲水性较强的多肽用极性较强的溶剂洗脱时效果较好，可用增加三氟乙酸的洗脱次数来提高这类多肽的洗脱率；