Methods: The synthesized BNP was conjugated to bovine serum albumin (BSA) by EDC coupling procedure to form complete antigen (BNP-BSA).

• 方法：合成的BNP与牛血清白蛋白(bovine serum albumin，BSA)以碳化二乙胺(EDC)法偶联，制备BNP-BSA完全抗原。