Methods: Gene fragment encoding alkaline protease was amplified by PCR from Bacillus subtilis A-109,sequenced and analyzed. The recombinant plasmid was constructed and transformed into E. coli BL21.SDS-PAGE detected the expression of the gene fragment.

• 方法:用PCR的方法从枯草芽孢杆菌A-109中扩增碱性蛋白酶基因(apr),并进行测序分析,构建表达载体,最后转化大肠杆菌BL21,SDS-聚丙烯酰胺凝胶电泳检测该基因的表达情况。