Methods The microsatellite polymorphism analyses were performed to detect the LOH on chromosome 6 and 8 by PCR and Perkin Elmer 377 DNA sequencer in the 21 cases with GBM.

• 方法 应用PCR方法和DNA序列自动分析仪,分析了21例GBM6号、8号染色体上20个、14个微卫星多态性标记的LOH。