Methods Immunohistochemistry and in situ hybridization technique were used to detect DCN protein and mRNA expressions in 30 cases of colorectal carcinoma tissues and 20 cases of relative normal tissues.

• 方法:采用原位杂交及免疫组织化学方法检测DCN mRNA及其蛋白在30例结直肠癌及20例相对正常大肠组织中的表达。