Method:Trypan blue exclusion, 3H|TdR incorporation and 3H|proline incorporation were assayed when LFb cultured in AM|conditioned medium on day 7 after bleomycin exposure. Western blot was carried out to determine the change of Ras process.

• 方法 :肺纤维化 1周组肺泡巨噬细胞 (AM)条件培养上清液与正常大鼠LFb共同培养 ,并给予不同浓度的Sim干预 ,利用3H 胸腺嘧啶核苷掺入法、3H 脯氨酸掺入法和Western杂交测定LFb增殖、胶原合成和Ras蛋白的翻译后修饰。