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Method:Tissue of stria vascularis was transferred from the cochlea to a cell-cultured dish using the explants culture technique. Explants were cultivated at 37C in a 5% CO2 incubator and examined daily by interted microscopy.
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采用移植培养技术,将活体分离的血管纹组织块接种于无菌塑料培养皿中,于5%25co2 37℃恒温箱内培养,每周换液2次,观察细胞生长情况。
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