Luciferase plasmid DNA and PEI were complexed to form slightly positive-charged nanoparticles, onto which FOL-PEG-PLL conjugate was surface coated.

• 首先用荧光素酶质粒DNA和PEI合成略带正电的纳米颗粒，再在其表面包裹FOL-PEG-PLL共轭化合物。