In vitro culture of Isospora developed slowly, which drawed back the research of Isospora in the field of endogenous developmental stages, drug screen, isospora attenuated and so on.

• 等孢球虫体外培养发展缓慢，严重制约着等孢球虫内生发育、药物筛选、虫株致弱等方面的研究。