In situ end-labeling (ISEL) was used in the light of instruction of Roche apoptosis kit to detect the apoptotic endothelial cells. The cells with brown karyon stained were deemed to be apoptotic cell under Olympus light microscope.

• 采用原位末端标记法,参照罗氏公司细胞凋亡检测试剂盒说明书检测治疗前后内皮细胞凋亡情况,OLYMPUS光学显微镜下观察,细胞核被染成红棕色的细胞视为凋亡细胞。