Identified by individual plant testing , analysis of RT-PCR and dot blot ,this fragment was only found in CMS cauliflower knxd612.Analysis of the sequence indicated it was high homologous(98%) with orf138 of Ogura CMS radish.

• 单株检测,RT PCR分析,斑点杂交鉴定,确定此片段为花椰菜细胞质雄性不育系 knxd612 所特有。 序列分析表明该片段与Ogura型胞质不育萝卜,不育相关开放读码框orf138的同源性高达98%25。