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- As leucine zipper is a basic DNA binding motif of many known transcription factors, we examined the transcriptional function of the TCP10L using dual luciferase assay system. 本研究的结果进一步加深了对人体MT基因结构与功能的认识,也为阐明HCC发生的分子机制提供了有重要价值的资料。
- Binding assay shown that Vp28 can not binding membrane protein of shrimp gill. 纯化的VP28与对虾鳃细胞膜的结合实验表明,VP28不能与对虾鳃细胞膜结合,即纯化的VP28不具有结合活性。
- The dopaminergic receptors were measured with the radio-ligand binding assay. 采用同位素标记法测定受体变化;
- The structure and expression pattern implies it migh t be a DNA binding transcription regulator. 它的结构和表达特征预示着它编码的是一个具DNA结合功能的转录调控因子.
- EMSA showed that stimulation of HSC by Aldo markedly increased AP-1 DNA binding activity. Aldo可增强HSC的AP-1的DNA结合活性。
- Competitive protein binding assay indicates the level of cAMP is improved when CDCA is given by oral administration. 通过竞争性蛋白质结合分析法测得,鹅去氧胆酸经灌胃给药后能显著提高大白鼠气管组织中cAMP的含量。
- The autoimmune regulator(AIRE) showned DNA binding activity and transcrip-tional transactivation potential. 自身免疫调节因子(autoimmune regulator,AIRE)是一种具有转录活化潜能的DNA结合蛋白。
- SSR was detected by radioligand binding assay(RBA) and ER, PR by LsAB method in these groups. 采用RBA法测定各组织的SSR表达 ,采用免疫LsAB法测定ER、PR表达。
- There were DNA binding proteins in the SR of rats, and the molecular weight was 78 000 and 60 000 dalton. 大鼠膈肌SR上存在非序列依赖性DNA结合蛋白 ,相对分子质量分别为 6 0 0 0 0道尔顿和 780 0 0道尔顿。
- The effects of l-CSL on recombinant D_1R were investigated by [3~H]SCH23390 binding assay and cAMP assay. [~3H]SCH23390受体结合和cAMP测定实验检测左旋氯代斯阔任对重组D_1受体的激动作用。
- These oligonucleotides contain consensus DNA binding sites for individual sequence-specific transcription factors. 这些寡核苷酸共同学列是单个序列特异性的转录因子DNA结合位点。
- Method H?3?cAMP was used in protein binding assay to determine the level of cAMP in bovine BTC. 方法运用蛋白竞争结合法用氚(H3)标记的环磷酸腺苷(adenosine3,5?cyclicmonphos?phate,cAMP)测定牛眼小梁细胞内cAMP的含量。
- The autoimmune regulator (AIRE) showned DNA binding activity and transcriptional transactivation potential. 自身免疫调节因子(autoimmune regulator, AIRE)是一种具有转录活化潜能的DNA结合蛋白。
- Methods: The number and affinity of IR on hepatocyte membrane of ASR were determined with radioligand binding assay. 采用放射配体结合分析方法检测肝细胞膜胰岛素受体的数目与亲和力。
- Methods Radioligand binding assay was used to determine the contents of GR and its affinity (Kd). 方法应用胃泌素受体的放射配基结合分析法,测定34例胃癌组织胃泌素受体的含量及其亲和力。
- DNA binding activity of transduction factor and transcription activator 3 between the two groups was similar ?P > 0.05?. 两组之间转导子和转录激活子3DNA结合活性接近(P>0.;05)。
- Telomeric proteins is composed of double DNA binding protein, single DNA binding protein and telomeric associated proteins. 端粒结合蛋白直接保护端粒DNA,端粒相关蛋白通过与端粒结合蛋白的相互作用间接影响端粒的功能。
- Competitive inhibition assay, saturation binding assay and scatchard analysis testify the expression of LH/hCG receptor. 竞争结合实验、受体结合的特异性实验、饱和结合实验证明3AO细胞上存在特异性的Ll壬七CG受体。
- All the results indicated that the structures of amino acid bridge in the ligands may affect the DNA binding and cleavage ability. 研究结果表明氨基酸桥对于配体的DNA键合及裂解能力的有不同影响。
- Methods: Wrap restraint stress induced defecation rats, the guanylin contents in colon and plasma were measured by radioligand binding assay. 方法:建立束缚应激诱发排便异常大鼠动物模型,应用放射配基法测定结肠组织及血浆中鸟苷素含量。
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