Conclusion This co-culture modelestablished by isolating highly purified EVCTs and DSCs in vitro can be useful for studying the trophoblast invasion mechanisms .

• 结论适当的培养方法可获得高纯度的EVCT和DSC,并使其维持特有的生物学活性,成功地建立了共培养系统模型,便于研究滋养细胞侵袭和胚胎着床的机制。