Coefficients of variation(CV)of ELISA in a lot and among the lots were 4.16% and 7.52% respectively,the P/N was 3.63 under the best experimental conditions determined by L 9(3 4)factorial design.

• EL ISA证实 HCV NS3- 4A蛋白酶能使特异底物裂解。 正交实验获得 EL ISA的最佳实验条件 ；其 P/ N值为 3.；6 3；批内和批间变异系数 (CV)分别为 4