At day 29 , the right Achilles tendons of all rats in both groups were harvested, prefixed in buffered formalin for 24 hours, and then embedded in paraffin.

• 第29天，获取每只大鼠的右侧跟腱，立即用4%25中性福马林固定，石蜡包埋，每个标本连续纵行切片，随机取其中部分切片行HE染色，观察跟腱组织病理改变，确认腱病模型是否成功（见第一部分）。