All the patients underwent ovarian stimulation and during 6 h after oocyte retrieval 1PBs were biopsied and WCP were used in FISH. On day 3 after fertilization embryos diagnosed as normal or balanced were transferred.

• 所有周期取卵后6h内通过活检取出第一极体,采用WCP探针进行荧光原位杂交,受精后第3天选择染色体组成正常或平衡的胚胎进行宫腔内移植。