coli strain BJ5183.The recombinant adenoviral constructs were cleaved with Pac I to expose their inverted terminal repeats (ITR) and transfected into 293 packaging cells to generate viruses.

• coli BJ5183细胞内进行腺病毒骨架质粒与克隆带有外源基因的穿梭载体同源重组,获得了重组腺病毒质粒DNA分子。