There isn't much difference in adventitious bud regeneration from leaves of proliferation of bud clusters or leaves of callus. 由丛生芽和愈伤组织两个途径得到的无菌苗上的叶片在再生能力方面没有明显差异.
Effects of TDZ on adventitious bud regeneration from leaf blade of apple[J].Plant Physiology Communication,1997,33 (6):420-423. TDZ对苹果叶片离体再生不定芽的效应[J].
No difference was found on the adventitious bud regeneration rates whether upper surfaces or lower surfaces of the leaves were touching the medium, but healthy calluses were generated from the latter. 叶块以近轴面或远轴面接触培养基的接种方式对不定芽再生影响不大，不过叶片远轴面贴近培养基接种，产生的愈伤组织状态较好；
Adventitious buds regeneration from etiolated internode segment of apple cultivar Nagafu No. 富士苹果黄化茎段再生不定芽的研究。
The leaf disks derived from the tip, middle, or base parts of the leaves had the same adventitious bud regenerate ability. 将叶片切分为叶尖、叶中、叶基部叶块分别接种，其不定芽再生能力没有差异；