When glucose is oxidized to glucolactone by glucose dehydrogenase (GDH, E.C. 1.1.1.47), NADP+ is reduced to NADPH, therefore, this reaction can be coupled to the reduction of COBE to (R)-CHBE.

• 利用微生物产酶催化还原COBE获得CHBE的过程需要辅酶NADPH参与提供氢原子。 由于工程菌E.