When cultured the AFP~+/Nestin~+/CK19~+ progenitor cells on Matrigel in William E culture medium supplemented with 100ng/ml HGF for 7 days, we observed bile canaliculi like structure in vitro.

• 将祖细胞培养在胞外基质Matrigel上,以含100ng/m1 HGF的William E培养液培养7d后观察发现祖细胞形成胆管上皮样结构。