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Western Blot was used to detect the ERK 1/2 levels and ERK activation product (P-ERK1/2) levels. Cell viabilities were measured with MTT assay. Cell cytopathic effect (CPE) was used to evaluate myocardial cell lesions.
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采用Westenblot分别测定ERK1/ 2 ,ERK激活后产物 (P ERK1/ 2 )表达变化 ,采用MTT法检测细胞活性 ,采用细胞病变法计算细胞病变。
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