We skillfully combinated PCR and reverse dot blot, using thehigh specificity of reverse dot blot overconming the deficiency ofhigh false positive rate of PCR as well as making use of thecharacteristic of high specificity of PCR.

• PCR-RDB检测方法将PCR技术和反向点杂交方法有机的结合起来，既利用了PCR技术灵敏度高的优点，又利用反向点杂交技术的高、度特异性克服了PCR方法假阳性率高的缺点，在实验室和现场的初步应用中都取得了较好的结果。