We reconstructed the gene of human scFv (VL-VH) and prepared scFv(VL-VH) and scFv (VH-VL) antibody fragments by prokaryotic expression system PET22b(+)/BL21(DE3).

• 对筛选获得的人源抗G5-24 scFv(VL-VH)基因进行了重构，将它们克隆入表达载体pET22b(+)中，转化大肠杆菌BL21(DE3) ，IPTG诱导表达；