WSSV(white spot syndrome virus) is isolated from infected crayfish cambarus clarki. , by different speed centrifugation , sucrose cushion , sucrose gradient centrifugation. Quantified by Bradford methods, the concentration of WSSV is 0.912mg/ml.

• 使用差速离心、蔗糖垫和蔗糖密度梯度离心法，从人工感染白斑综合症病毒(White spot syndrome virus，WSSV)的克氏原螯虾中提取WSSV，Bradford法测定病毒浓度为0.；912mg/ml。