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VSMCs were lysed and the concentration of protein was measured using Coomassie blue G250 staining technique. PDGFR- 3 protein expressions were determined by Western Blotting.
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时,裂解细胞提取蛋白,考马斯亮蓝G一250染色法测定蛋白浓度,使用Westem Bloning法检测PDGFR一p蛋白表达量。
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