Using the total RNA extracted from ConA stimulated equine peripheral blood mononuclear cells(PBMC) as template,the cDNA of interleukin l8 was amplified by RT-PCR. The cDNA was subsequently cloned into the vector pCR2.1-TOPO and sequenced.

• 用RT-PCR从经ConA刺激的马外周血单个核细胞(PBMC)中扩增出马白细胞介素18(Equine interleu-kin-18；EIL-18)前体蛋白(precursor EIL-18；pEIL-18)基因的cDNA；然后克隆至载体pCR2.；1-TOPO中；鉴定并命名为pCR2