Using prue culture of cells of SAN, immunohistochemical and immunocytochemical reaction, immunoelectron microscope (pAg technique) and image analysis methods, the ANF expression in SAN cells of neonatal SD rats was studied.

• 本实验采用窦房结细胞纯化培养、免疫细胞化学、免疫组化、免疫电镜 （pAg 技术） 和图像分析等多种方法，研究了新生SD大鼠窦房结的ANF表达。