Urinary sample was hydrolyzed by base, then extracted by solvent and cleaned by silica gel column.Afterwards, the sample was purified by high performance liquid chromatograph (HPLC).

• 尿样经碱水解、溶剂提取及硅胶柱净化后,用高效液相色谱仪纯化,纯化液作荧光光谱及气相色谱/质谱分析;