Totally 30 proestrus female and adult male SD rats were divided into 3 groups: female group, male group and sham operation group with 10 rats in each group, of which there were 5 male and 5 female rats in sham operation group.

• 实验动物为发情前期的雌性和成年雄性SD大鼠30只,分为雄性,雌性和假手术3组,每组10只,其中假手术组雌雄各5只。