To understand the mechanism of ganglioside GM 3 effecting on protein kinase C (PKC), the translocation of PKC isoforms in J6 2 cells and the content of diacylglycerol (DAG) was compared between cells treated with GM 3 and control cells.

• 应用SDS PAGE及Western印迹技术检测神经节苷脂GM3 处理前后人白血病J6 2细胞不同类型PKC在细胞内的转位情况 ；同时利用高效薄板层析技术观察了细胞内DAG含量的变化 ；从而探讨GM3 抑制PKC活性的机制 .