To duplicating the regulatory subunit p35Nck5a gene of mouse neuronal cdc2-like kinase in embryonic stem (ES) cells, about 12.2kb of pGDTV vector for p35Nck5a gene duplication was constructed.

• 为了在小鼠胚胎干细胞（ES）中引起神经细胞cdc2类激酶调节亚基p35Nck5a基因的定点重复，采用常规的分子克隆技术，构建得到长约12.；2kb的基因重复性打靶载体pGDTV。