Tie template RNA of Cucumber mosaic virus 2-1-2 isolated from Echinacea purpurea Moench was amplified by RT-PCR,the amplified fragments were cloned and sequenced.

• 以紫松果菊上分离的黄瓜花叶病毒2-1-2的RNA为模板,采用RT-PCR(反转录聚合酶链式反应,下同)技术对2-1-2的外壳蛋白(coat prote in,CP)基因进行了克隆和序列测定。