Though HCV RNA amplification by PCR is the most sensitive and specific method for the detection of HCV, the complicated manipulation, easy contamination and high cost prevented PCR from application in a large scale.

• PCR检测HCV RNA是目前HCV诊断最灵敏、特异的方法，但其操作比较复杂，易造成污染，且价格昂贵，不适于大规模的筛查应用。